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Object-oriented programming sounds ominous at rst, but it s simply a way of looking at things. Even if you ve never done any programming at all, you re already familiar with the way it works, because it s simply an expression of the way the real world works. The book in your hands is an object. It contains other objects such as chapters, illustrations, example code, and so forth. Similarly, a Web browser is an object that contains other objects that are represented by HTML elements. When you re using JavaScript, a Web browser is called a window object. The Web page it contains is known as a document object. If there is an image in the Web page, it s an image object. You get the idea it s all just another way of stating what s showing up in the browser and what s on the page. Objects are referenced from the top down, starting with the window object, and each object is separated from the next by a period. Thus, the Web page is referenced as window.document. If it contains an element named sidePanel, that form can be referenced as window .document.sidePanel. It s just as if you re going down a stairway one step at a time.

Although linear trendlines are most common, an Excel chart can display nonlinear trendlines of the following types:

To call CFC functions from a Flex application, follow these steps:

To insert your modified Flash button, follow these steps:

The third line of code will execute only if the if condition on the second line is true. Here, we enable the dragging behavior of the position instance by using the startDrag method on this. Because it s used as a method and not as an action, we don t need to specify a target instance in the arguments. The arguments prescribed here lock the mouse to the center of the object and constrain the draggable region to a bounding box defined by 10, 0 and 200, 0. This effectively keeps the position instance confined to the line of our sliderRule Graphic.

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The libs folder, which will initially be empty, can contain libraries of SWC files. SWCs are precompiled components that can be used across multiple Flex projects.

3-D object pop-up (Layout mode only): This control enables you to add 3-D embossing, engraving, or a drop shadow to any selected object. Zoom percentage (all modes): This box shows the current zoom level. (Zooming enlarges or shrinks your view of what s onscreen.) Click the box to switch between the current zoom level and 100 percent. Zoom controls (all modes): Click the left icon to reduce the view to a bird s-eye perspective. Click the right icon to increase the document s zoom (magnification) level. Status area control (all modes): Click this control to show or hide the status area (on the left side of the window), including all tools. Part label control (Layout mode only): Click this control to switch between displaying layout part labels horizontally and vertically.

5 f (GHz)

(c) Transfer cells to a 15-mL conical tube containing 6 mL of prewarmed FCM, mix brie y by gentle pipetting, and centrifuge at 250 g for 3 min. (d) Resuspend cells in FCM to half the intended total culture volume; dispense the other half volume to the culture plates or wells; proportion the resuspended feeders evenly across the plate(s) or wells. Carefully place the culture vessels in the cell culture incubator. (e) The following morning (i.e., the day of tissue collection), aspirate the FCM and gently wash the cells with sterile PBSA. (f) Remove the PBSA and gently add prewarmed GCM to half of the intended total gonad cell culture volume (see Protocol 5.5). (g) Return culture vessels to the incubator until the time of plating the gonadderived cells.

Using Other Navigator Containers ....................................................................................497 The TabNavigator container ....................................................................................498 The Accordion container .........................................................................................500 TabNavigator and Accordion keyboard shortcuts ....................................................501 Summary ..........................................................................................................................502

The basic crystal function of converting gamma photon energy into light photon energy is discussed in 5. Thallium-doped sodium iodide (NaI(Tl)) crystals were originally used for PET systems; however, NaI has a relatively low density and is less effective at stopping the highenergy 511-keV photons. To compensate for the lower density, thicker crystals were employed for 511-keV imaging than those used for detecting

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